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Image Search Results
Journal: bioRxiv
Article Title: Rapid acquisition of internal models of timing by Purkinje cells enables faster behavioral responses
doi: 10.1101/2021.04.28.441782
Figure Lengend Snippet: (A) Schematic of the custom built experimental setup. A head restrained 7dpf zebrafish larva is placed in a closed-loop optomotor track underneath a two-photon microscope. L1-6: convex lenses, F1-3 optical filters (See Methods). (B) A cartoon of the experimental protocol. Each trial is 2s long followed by a 5s inter-trial interval. 10% of trials chosen pseudorandomly are probe trials in which optic flow is not turned on. Latency to respond to optic flow in each trial is measured as the behavioral readout. (C) Average image of a single optical plane of Purkinje cells specifically expressing GCaMP6s under the aldoca promoter. Scale bar: 20µm. (D) A video grab from the closed-loop camera showing the tail of a head restrained fish while two-photon imaging was being carried out. (E) Example calcium imaging traces (blue) from 5 cells (ROIs marked in (C)) aligned to motor output (black trace) and the optic flow stimulus (green trace). (F) Representative behavioral response during a 100-trial experiment (top) and a zoomed-in section showing swim bouts around a single probe trial (bottom). (G) Most swim bouts occur during optic flow presentation. Almost no swim bouts occur during probe trials or in the interval between trials. p = 1.01 x 10 -13 by one-way repeated measures ANOVA, 27 fish. **p = 0.001 by Tukey’s post hoc test. (H) Average latency distribution for 27 fish (1.15 ± 0.29, mean ± SD).
Article Snippet: The pipettes were guided visually using the 60x/1.0NA water immersion objective of a
Techniques: Microscopy, Expressing, Imaging